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Pathology and Laboratory Medicine
Test Catalogue

​Culture and Sensitivity (C&S) Cerebrospinal Fluid

Clinical Microbiology

​Ordering Recommendations

​Specimen Requirements​

  • AV reservoir/shunt 
  • Lumbar puncture/drain fluid 
  • Ventricular fluid
​Collection Procedure
  • Refer to CSF Collection Guidelines for order of draw instructions

  • Tubes provided in the Lumbar Puncture Tray (CSF Collection Kit) are pre-numbered (1 to 4) indicating the order sample tubes are drawn during collection. IF alternate collection container is used, tubes MUST be numbered with the order sample tubes are drawn.

​Required Volume
  • Optimal Volume: 1.0 mL (Adults & Children)
                                  : 0.5 mL (Neonates)
    (Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory/reflex testing. In some situations, a minimum volume may require a second collection.)
  • Hand deliver to laboratory within 15 minutes of collection
  • Stable at room temperature
  • Do NOT refrigerate or freeze
  • Specimen must be received in laboratory within 24 hours of collection
Grounds for Rejection​

Testing Information

Testing Site​
  • ​Bacteriology Laboratory, RUH
Results Reporting​
​Clinical Interpretation
​Specimen Retention

Test Ordering Requirements​

Forms Required


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Laboratory Controlled Document  LSM 814 V1

Last Modified: Friday, September 27, 2019 |
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