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Pathology and La
Culture and Sensitivity (C&S) Eye/Other

Clinical Microbiology

​Ordering Recommendations

Specimen #1 Requirements​ ​

Type​
  • Conjunctiva Swab

​Container

​Stability/Storage
  • Transport within 2 hours of collection for optimal organism recovery
  • Stable at room temperature up to 24 hours
  • Do NOT refrigerate
  • Specimen must be received in laboratory within 24 hours of collection
Grounds for Rejection​

Specimen #2 Requirements​ ​

​Type
  • Corneal Scrapings
​Container

  • Preferred Collection Container: Direct culture media inoculation

​Collection Procedure
​Stability/Storage
  • Transport within 1 hour of collection for optimal organism recovery
  • Stable at room temperature up to 24 hours
  • Do NOT refrigerate
  • Specimen must be received in laboratory within 24 hours of collection
​Grounds for Rejection

​ ​Specimen #3 Requirements

​Type​Eye fluid (Aqueous or Vitreous)
​Container​Preferred Collection Container: Sterile container WITHOUT transport media, additives or preservatives
​Collection Procedure​If specimen is being sent in syringe used for collection, needle MUST be removed and syringe aseptically sealed with a sterile syringe cap
​Required Volume
  • ​Optimal Volume: Entire collection 
  • Minimum Volume: 0.1 mL
    (Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory/reflex testing. In some situations, a minimum volume may require a second collection)
​Stability/Storage
  • Transport within 1 hour of collection for optimal organism recovery
  • Stable at room temperature up to 24 hours
  • Do NOT refrigerate
  • Specimen must be received in laboratory within 24 hours of collection
​Grounds for Rejection

Testing Information​ ​

Availability​
Testing Site​
  • ​Bacteriology Laboratory, RUH
Results Reporting​
Clinical Interpretation
​Specimen Retention

​Alternate Test Names
  • Conjunctivitis
  • Keratitis

Test Ordering Requirements​ ​

​Forms Required
​Clearance Required​Conjunctival swabs collected under special circumstances (i.e. persistent infection or treatment failure, blepharitis, or corneal ulcers) must be cleared the Microbiologist on-call at (306)655-1000 prior to processing


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Laboratory Controlled Document LSM-980 v1

Last Modified: Friday, September 27, 2019 |
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