Anti-Nuclear Antibodies

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Anti-Nuclear Antibodies

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Clinical Microbiology

Specimen Requirements
Type	Serum Body Fluid (Cerebrospinal, Pericardial, Peritoneal, Pleural, Synovial)
Container	Whole Blood Preferred Collection Container: Red Alternative Collection Container: Tiger (gel) Body Fluid Preferred Collection Container: Sterile, wide mouth screw cap Alternative Collection Container: Sterile, black screw top round bottom tube
Required Volume	Whole Blood Optimal Volume: 4.0 mL Pediatric Volume: 3.0 mL Minimum Volume: 2.0 mL (Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory/reflex testing. In some situations, a minimum volume may require a second collection) Body Fluid Optimal Volume: 1.0 mL (spun) Minimum Volume: 0.5 mL (spun) Serum (For Laboratory Use Only) Minimum Volume: 0.5 mL
Stability/Storage	Body Fluid Stable at room temperature for 1 hour Greater than 1 hour, refrigerate at 2°C to 8°C prior to transport Serum Serum must be removed from cells or gel within 4 hours of collection Stable up to 7 days at 2°C to 8°C
Grounds for Rejection	Samples with repeated freeze/thaw cycle Samples held at ambient temperature for greater than 24 hours Pathology and Laboratory Medicine Acceptance and Rejection Criteria
Testing Information
Availability	Division of Clinical Microbiology Laboratory Hours of Operation STAT requests outside of these hours must be approved by the Microbiologist-on-call at 306-655-1000
Testing Site	Immunodiagnostics Laboratory, RUH
Results Reporting	4 days
Methodology	Indirect Immunofluorescence (IFA)
Specimen Retention	1 month
Alternate Test Names	Anti-nuclear antibody screening test Anti-Nuclear Antibodies (ANA)
SHR LIS Test Code	ANA (For Laboratory Use Only)
Test Ordering Requirements
Forms Required	Community Laboratory Requisition Form #101064

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Laboratory Controlled Document LSM-468 v1

Last Modified: Tuesday, February 2, 2016 |

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​Anti-Nuclear Antibodies

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Clinical Microbiology

Specimen Requirements​ ​

Testing Information​ ​

Test Ordering Requirements​ ​

Anti-Nuclear Antibodies

Specimen Requirements

Testing Information

Test Ordering Requirements