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Pathology and Laboratory Medicine
Test Catalogue

​bcl-1/JH, t(11;14) Translocation by PCR (bcl1)

Human Molecular Genetics


​ ​Specimen Requirements

Type​
  • ​Submit only one of the following specimens as appropriate:
    • Bone marrow aspirate
    • Peripheral blood
    • Body fluid
    • Fresh or frozen tissue
    • Formalin fixed, paraffin embedded tissue
​Container
  • ​Preferred Collection Container:
    • Bone marrow aspirate: 4 ml Lavender (K2 EDTA)
    • Peripheral blood: 4 ml Lavender (K2 EDTA)
    • Body fluid: sterile tube, vial, container
    • Fresh or frozen tissue: clean container
    • Formalin fixed, paraffin embedded tissue: paraffin block
​Collection Procedure
  • ​Collect as appropriate for specimen type
  • Fresh specimens should be transported to Royal University Hospital, Human Molecular Genetics
​Required Volume
  • Optimal Volume:
    • ​Bone marrow aspirate: 1 ml
    • Peripheral blood : 1 ml
    • Body fluid: 1 ml
    • Fresh or frozen tissue: 100 mg
​Stability/Storage
  • ​Ship all but frozen specimens at ambient temperature
  • Bone marrow, peripheral blood and fluids may be stored at 2 - 8°C
Grounds for Rejection​

Testing Information​ ​

Availability​
  • ​Testing performed Monday to Friday, 8:00 a.m. to 4:30 p.m.
  • STAT testing can be requested but is only available Monday to Friday, days
Testing Site​
  • Royal University Hospital, Human Molecular Genetics Laboratory
Results Reporting​
  • ​Results are generally available within 7 working days
  • STAT results are generally available within 3 working days
​Methodology
  • ​DNA is extracted and PCR amplified using primers targeting the major translocation
    cluster (MTC-1 and MTC-3) of the bcl-1 locus and the immunoglobulin heavy chain
    (IgH) gene joining region (JH)
  • However, some breakpoints occur at distance loci (e.g. PRADI site) and will not be identified by this particular test
​Clinical Interpretation
  • ​Presence or absence of the bcl-1/JH t(11;14)
​Specimen Retention
  • ​Peripheral blood retained for 14 days
  • Bone marrow retained for 1 month
  • Paraffin blocks are stored for 20 years (Anatomic Pathology guidelines)
  • Fresh tissues and fluids are retained for 1 week
  • Extracted DNA stored for 2 years
Alternate Test Names​
  • Bcl-1/JH t(11;14)(q13;q32) amplification
  • Mantle cell lymphoma PCR
  • Bcl-1
​Additional Comments
  • ​Successful PCR is dependent on the yield and quality of the extracted DNA
  • Occasionally, and particularly in paraffin-embedded tissues, DNA yield and quality
    may not be adequate for successful testing
  • This test is not intended for minimal residual disease detection

Testing Ordering Requirements​ ​

Clearance Required
  • ​Requests for bcl-1/JH translocation analysis must be made after consultation with the
    Hematopathologist on call



​If you choose to print this information, it is valid only on date of print.

Laboratory Controlled Document LSM-332 v3

Last Modified: Thursday, October 3, 2019 |
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