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Pathology and Laboratory Medicine
Test Catalogue

​Blood and/or Bone Marrow Aspirate

Hematology - Flow Cytometry

​Ordering Recommendation

  • ​ ​Performed on suspected Acute Leukemia, lymphocytosis or when abnormal lymphoid cells present

Specimen Requirements​ ​

  • ​Blood
  • Bone Marrow Aspirate
  • 4 unstained smears on specimens collected outside Saskatoon
​Collection Procedure
  • Specimens may be collected Sunday to Thursday, must be less than 24 hours old when received in the Flow Cytometry Lab
  • Specimens must arrive before noon on Friday (or day before Stat holiday)
  • Referred-in specimens (specimens not collected with-in Saskatoon) phone 655-2220 to notify lab prior to collection
  • If you require a Flow Cytometry Requisition we will fax or e-mail one to you. Complete requisition with patient’s name, sex, HSN, Doctor, diagnosis and relevant clinical information.
  • Include recent CBC and differential result
  • Package requisition, CBC and differential results, and smears with sample(s) in an insulated container with ice pack. Insulate specimen from ice pack to protect from freezing. Put address labels on two sides of the package or box
  • Example of address label:

St. Paul’s Hospital
Laboratory – Specimen Management
1702 20th Street West
Saskatoon SK S7M 0Z9

*Deliver upon arrival – phone GDML Courier 291-7501
Attention: Flow Cytometry specimen

​Required Volume
  • ​Optimal Volume:
    • Blood- 2 – 4mL

    • Bone Marrow Aspirate – 1- 4 mL
      (Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory/reflex testing. In some situations, a minimum volume may require a second collection.)

  • ​Test within 24 hours of collection
Grounds for Rejection​

Testing Information​ ​

  • ​Monday to Thursday 0730 to 1600, Friday until 1200 excluding Statutory holidays unless approved by the Hematopathologist on call
Testing Site​
  • ​RUH Hematology-Flow Cytometry
Results Reporting​
  • ​3 working days
Alternate Test Names​
  • ​Immunophenotyping
  • Surface cell markers
  • T and B subsets
​Additional Comments
  • ​If Bone Marrow aspirate is not possible, Flow Cytometry can be performed on Blood provided greater than 10% blasts are present
  • Flow Cytometry is not performed for neutrophilia in blood
  • If Bone Marrow differential/Biopsy is also required, phone RUH Hematology Laboratory306-655-2191. Instructions for Bone Marrow procedure are available at this number.
  • For Cytogenetic information phone 655-1706
SHR LIS Test Code​
  • ​FLOWC (for Laboratory Use Only)

​ ​Test Ordering Requirements

​Forms Required

​ ​Patient History

​Relevant Clinical History
  • ​Include on requisition any Clinical details and/or diagnosis


​If you choose to print this information, it is valid only on date of print.

Laboratory Controlled Document LSM-24 v2

Last Modified: Thursday, December 14, 2017 |
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