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Pathology and Laboratory Medicine
Test Catalogue

​Chromosome Analysis - Amniotic Fluid


​Ordering Recommendation

  • ​ ​Optimal timing for specimen collection is between 14 to 18 weeks of gestation, but specimens collected at other weeks of gestation are also accepted

​ ​Specimen Requirements

  • ​Amniotic Fluid
  • ​Preferred Collection Container: 2 sterile 15 ml tubes such as Corning or BD
​Collection Procedure
  • ​Specimen must be accompanied by a completed test requisition
  • Failure to include relevant patient information may result in test results being delayed or discontinued
​Required Volume
  • ​Optimal Volume: 15-18 ml divided between two tubes
  • Minimum Volume: All samples are accepted regardless of volume
    (Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory/reflex testing. In some situations, a minimum volume may require a second collection.)
  • ​Specimens are to be kept and delivered at room temperature
  • Do not freeze specimen or expose to extreme heat
Grounds for Rejection​

Testing Information​ ​

  • ​Specimens are received in the Cytogenetics Laboratory weekdays from 8:00 a.m. to 5:00 p.m.
  • There is a Cytogenetics technologist on call Saturday, Sunday and statutory holidays from 8:30 a.m. to 5:00 p.m. for urgent specimens only. This person can be reached through Royal University Hospital Switchboard (655-1000)
Testing Site​Cytogenetics Laboratory
Ellis Hall Ground Floor, Room 35
Royal University Hospital, 103 Hospital Drive
Saskatoon SK S7N 0W8
Phone: 306-655-1706
FAX: 306-655-6462
Results Reporting​
  • ​14-21 days, dependent on indication, growth rate and workload
​Reflex Testing
  • ​Parental chromosome analysis may be indicated to clarify a chromosome
    rearrangement and determine recurrence risk
  • The fluorescence in situ hybridization (FISH) methodology may be used to further
    define a rearrangement
  • ​Amniocyte long-term culture and G-banding
​Clinical Interpretation
  • ​Diagnosis of constitutional chromosome anomalies including numerical and structural anomalies
  • Anomaly designation follows the most recent edition of the International System for Human Cytogenetics Nomenclature
  • It is recommended that a qualified professional in Medical genetics communicate all abnormal results to patients
​Specimen Retention
  • ​Culture vessels: two weeks after reporting
Alternate Test Names​
  • Genetic Amniocentesis
  • Cytogenetic analysis
  • Karyotype
​Additional Comments
  • ​Molecular genetics (DNA) testing is done through referral to Medical Genetics
SHR LIS Test Code​
  • AF, RF (For Laboratory Use Only)

Testing Ordering Requirements​ ​

​Forms Required
  • ​Cytogenetic Prenatal Diagnosis Requisition obtained from the Cytogenetics Laboratory

Patient History​ ​

​Relevant Clinical History
  • ​Positive maternal serum screen
  • Ultrasound anomalies
  • Advanced maternal age (> 40 years)
  • Previous pregnancy with a chromosome anomaly
  • Family history of a chromosome anomaly
  • Fetus at risk for a genetic disease previously characterized in the parents (patient must be referred through Medical Genetics)

​If you choose to print this information, it is valid only on date of print.

Laboratory Controlled Document LSM-158 v1

Last Modified: Wednesday, February 3, 2016 |
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