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Pathology and Laboratory Medicine
Test Catalogue

​Cold Screen for Cardiovascular Surgery

Transfusion Medicine

​Ordering Recommendation

  • ​This test is only for patient’s undergoing cardiovascular surgery using bypass equipment
  • Not indicated as a screening test for cold agglutinin disease

​Specimen Requirements​

  • ​Whole blood
​Required Volume
  • ​Optimal Volume: 4 mL
  • Minimum Volume: 1 mL
    (Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory/reflex testing. In some situations, a minimum volume may require a second collection.)
  • ​Room temperature 20-24°C
Grounds for Rejection​

Testing Information​ ​



  • RUH on site testing available 24 hours/7 days per week
  • SCH on site testing available 0730-1530 Monday through Friday, excluding statutory holidays and bank days. After hours (1531 through 0729 hours) testing referred to RUH.
  • SPH on site testing available 0700-1730 Monday through Friday, excluding statutory holidays. After hours (1731through 00659 hours) testing referred to RUH.


  • Not available
Results Reporting​


  • Within 24 hours of receipt in Transfusion Medicine


  • Within one hour of receipt in Transfusion Medicine
​Clinical Interpretation
  • ​Detects cold agglutinins present in the patient which may cause complications during cardiovascular surgery due to the lowered body temperature
​Specimen Retention
  • 5 weeks, however testing must be performed prior to storage at <20°C
Alternate Test Names​
  • ​Cold screen
  • Cold agglutinins

​Additional Comments

​When indicated, cold antibody screen investigation for select patients scheduled to undergo cardiovascular surgery shall be performed no more than twenty eight days prior to surgical date.

SHR LIS Test Code​

  • ​BBSPC (For Laboratory Use Only)

Test Ordering Requirements​ ​

​Forms Required


​If you choose to print this information, it is valid only on date of print.

Laboratory Controlled Document LSM-43 v2

Last Modified: Wednesday, May 8, 2019 |
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