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Pathology and Laboratory Medicine
Test Catalogue

​Factor 5 Leiden Nucleotide 1691 Gene Mutation (F5L)

Human Molecular Genetics


​Ordering Recommendation

  • Can be ordered singly as Factor 5 Leiden (F5L) or is included in group code THROM (thrombophilia investigation). One tube will suffice for both molecular tests (F5L and PR202)
  • Repeat testing is not performed since this is a genomic DNA test

​ ​Specimen Requirements

Type​
  • ​Whole Blood
​Container
​Collection Procedure
  • ​Specimens sent for Factor 5 Leiden shall be drawn by venipuncture
  • No special instructions for patient before collection
  • Specimens should be transported to Royal University Hospital, Human Molecular Genetics without unnecessary delay
​Required Volume
  • ​Optimal Volume: 4.0 ml
  • Minimum Volume: 1.0 ml
​Stability/Storage
  • ​Ship specimens at ambient temperature
  • Specimens may be stored at 2 - 8°C
Grounds for Rejection​

Testing Information​ ​

Availability​
  • ​Routine batch testing performed Monday to Friday, 8:00 a.m. to 4:30 p.m.
  • Not a STAT test
Testing Site​
  • Royal University Hospital, Human Molecular Genetics Laboratory
Results Reporting​
  • ​Maximum turn around time 14 days from specimen receipt
​Methodology
  • ​Allele specific PCR
​Clinical Interpretation
  • ​Detects if the sample is positive (heterozygous or homozygous for this specific F5 mutation) or negative (homozygous wild-type)
​Specimen Retention
  • ​Peripheral blood retained for 14 days
  • Extracted DNA stored for 2 years
Alternate Test Names​
  • F5L
  • Factor V Leiden
  • FVL
  • Part of the Thrombophilia Investigation (group code THROM)
​SHR LIS Test Code
  • ​F5L or [included in thrombophilia investigation (group code THROM] (For laboratory use only)

Testing Ordering Requirements​ ​

Forms Required



​If you choose to print this information, it is valid only on date of print.

Laboratory Controlled Document LSM-335 v3

Last Modified: Monday, September 9, 2019 |
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