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Pathology and Laboratory Medicine
Test Catalogue

​Factor 5 (Factor V)

Hematology - Special Coagulation

​Ordering Recommendation

  • ​​Patient should not be on any oral anticoagulants. If unable to discontinue anticoagulant therapy, suggest consultation with Hematopathologist prior to ordering
  • This is not a Factor V Leiden; see Molecular Pathology

Specimen Requirements​ ​

  • ​Whole blood
  • ​Preferred Collection Container: Blue plastic (3.2% Buffered Na citrate)
  • Collection tube must be filled until the vacuum on the tube is completely exhausted to maintain an anticoagulant : blood ratio of 1:10
​Collection Procedure
​Required Volume
  • ​When testing multiple factors please phone Special Coagulation
    (306-655-2178) for optimum volumes
  • Optimal Volume: Two 2.7ml 3.2% Buffered Na Citrate or Three 1.8 ml 3.2% Buffered Na Citrate
    (Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory/reflex testing. In some situations, a minimum volume may require a second collection.)
  • ​Whole blood specimen shall be received at RUH Coagulation Laboratory within 2 hours of collection
  • Specimen is discarded after reporting. If an extra specimen is collected it is retained for 3 months.
Grounds for Rejection​
  • ​Line draws
  • Hemolyzed
  • Clotted
  • Inappropriately filled tubes
  • Patient on anticoagulant therapy testing must be approved by the Hematopathologist on service
  • Frozen aliquot specimen shipped without dry ice
  • Plasma specimen that has thawed and been refrozen
  • Plasma specimen that has thawed for more than 2 hours
  • Pathology and Laboratory Medicine Acceptance and Rejection Criteria   

Testing Information​ ​

  • ​Monday to Friday 07:30 – 16:00 excluding statutory holidays unless approved by the hematopathologist on call
Testing Site​
  • ​RUH Special Coagulation Laboratory
Results Reporting​
  • ​Specimens are batched and performed once per week
SHR LIS Test Code​
  • F5 (For Laboratory Use Only)


​If you choose to print this information, it is valid only on date of print.

Laboratory Controlled Document LSM-272 v3

Last Modified: Monday, February 3, 2020 |
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