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​Fluorescence in situ Hybridization (FISH) - Bone Marrow

Cytogenetics


​Ordering Recommendation

  • ​​Specimens are accepted on any business day and on weekends if urgent
  • Peripheral blood specimens are acceptable if there is greater than 10% blasts or for an indication of chronic lymphocytic leukemia

​ ​Specimen Requirements

Type​
  • ​Bone Marrow
  • Peripheral blood
​Container
  • ​Preferred Collection Container: Sodium Heparin (dark green top)
    • Note: Lithium Heparin tubes also have a green top and are NOT the proper tube to use
  • Sterile tube with transport medium
​Collection Procedure
  • ​Invert several times to mix
  • Other anticoagulants are not recommended since they are harmful to the viability of the cells
  • Specimen must be accompanied by a completed test requisition
  • Failure to include relevant patient information may result in test results being delayed or discontinued
​Required Volume
  • ​Optimal Volume: 1-3 ml of bone marrow or 4-7 ml of blood
  • Minimum Volume: 1 ml
    (Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory/reflex testing. In some situations, a minimum volume may require a second collection.)
  • All samples are accepted regardless of volumes
​Stability/Storage
  • ​Specimens are to be kept and delivered at room temperature
  • Do not freeze specimen or expose to extreme heat
Grounds for Rejection​

Testing Information​ ​

Availability​
  • ​Specimens are received in the Cytogenetics Laboratory weekdays from 8:00 a.m. to 5:00 p.m.
  • There is a Cytogenetics technologist on call Saturday, Sunday and statutory holidays from 8:30 a.m. to 5:00 p.m. for urgent specimens only. This person can be reached through Royal University Hospital Switchboard (655-1000)
  • Booking of specimens from outside the Saskatoon Health Region is recommended for tracking purposes
Testing Site​Cytogenetics Laboratory
Ellis Hall 5th Floor, 1955 building
Royal University Hospital, 103 Hospital Drive
Saskatoon SK S7N 0W8
Phone: 306-655-1706
FAX: 306-655-6462
Results Reporting​
  • ​Dependent on specimen type, indication and workload
  • STAT and urgent specimens are reported within 14 days
​Reflex Testing
  • ​Chromosome analysis
​Methodology
  • ​Unstimulated bone marrow/blood culture or direct preparation and fluorescence in situ (FISH) hybridization with commercially available targeted probes
​Clinical Interpretation
  • ​Diagnosis of acquired hematological disorders
  • Anomaly designation follows the most recent edition of the International System for Human Cytogenetic Nomenclature
​Specimen Retention
  • ​Original bone marrow or blood specimen: two weeks
  • Cell suspension: three months after reporting
  • FISH slide(s): one year
Alternate Test Names​
  • FISH
​Additional Comments
  • Solid tumors, lymph nodes and paraffin blocks are not accepted
  • Bone Marrow or blood smears can be accepted if no other alternative

Testing Ordering Requirements​ ​

​Forms Required

Patient History​ ​

​Relevant Clinical History
  • ​Hematological Malignancies
  • Lymphoma- to check for bone marrow involvement
  • Follow-up post-induction or post-transplant
  • The laboratory has a probe menu according to indication. Please inquire if needed


​If you choose to print this information, it is valid only on date of print.

Laboratory Controlled Document LSM-164 v2

Last Modified: Monday, October 7, 2019 |
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