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Pathology and Laboratory Medicine
Test Catalogue

​Group and Screen

Transfusion Medicine

​Specimen Requirements​

Type​
  • ​Whole blood
​Container
  • ​Preferred Collection Container: Lavender (EDTA)
    • Microvette EDTA is not acceptable
​Required Volume

Saskatoon and Humboldt and Rosthern:

  • ​Optimal Volume: 4 mL
  • Minimum Volume: 2 mL
    (Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory/reflex testing. In some situations, a minimum volume may require a second collection.)
  • Pediatric Volume: 1 mL

Transfuse-only SHR sites or referred-in specimens:

​Stability/Storage
  • ​Store at 2 - 8º C
  • Must be tested within 96 hours of collection
Grounds for Rejection​

Saskatoon and Humboldt and Rosthern:

Transfuse-only SHR sites or referred-in specimens:

Testing Information​ ​

Availability​

​Saskatoon:

  • RUH on site testing available 24 hours/7 days
  • SCH on site testing available 0730-1530 Monday through Friday, excluding statutory holidays and bank days. After hours (1531 through 0729 hours) testing referred to RUH.
  • SPH on site testing available 0730-1930 Monday through Friday, excluding statutory holidays. After hours (1931 through 0729 hours) testing referred to RUH.

Humboldt:

  • On site testing available 0700-1730 Mon-Fri, 0800-1600 weekends/stats, call-back after hours
Transfuse-only SHR sites or referred-in specimens: Not performed in local laboratory. Collected specimens must be sent to Saskatoon for testing.
  • Routine samples processed 0700-1630 Monday through Friday, excluding statutory holidays and bank days
  • Requests for urgent or after-hours sample processing must be discussed and approved by the Transfusion Medicine Physician on-call
Results Reporting​

Routine:

  • Saskatoon/Humboldt: within 24 hours of receipt in Transfusion Medicine
  • Transfuse-only SHR sites: within 48 hours of receipt depending on complexity of testing

Urgent:

  • Saskatoon/Humboldt: within four hours of receipt in Transfusion Medicine
  • Transfuse-only SHR sites or referred-in specimens:  not available

STAT:

  • Saskatoon/Humboldt – within one hour of receipt in Transfusion Medicine
  • Referred in  – not available

Note: Delays in reporting may be encountered in the presence of unexpected antibodies

​Reflex Testing
  • ​Minimum testing includes ABO group, Rh type, and indirect antiglobulin test for antibody screen
    • Humboldt: positive antibody screens are referred to Saskatoon
    • Direct antiglobulin test will be included on initial group and screen when patient is less than 4 months of age
  • Reflex testing is determined by results of initial testing and patient clinical circumstances
​Clinical Interpretation
  • ​If there is any discrepancy of ABO group test results, crossmatched group O red blood cells will be issued until the discrepancy is resolved
  • A positive antibody screen in may necessitate further testing to inform optimal blood component or product transfusion decisions. Contact the Transfusion Medicine Physician if assistance is required. 
Alternate Test Names​
  • ​Type and Screen
  • Antibody screen
  • Indirect Antiglobulin test
  • Prenatal Group and Screen
  • Prenatal Group and AB screen
  • T&S
  • G&S
​Additional Comments
  • ​Required every 96 hours
    • In Saskatoon: This may be extended during the current hospital admission if patient has not been transfused or pregnant in the last 3 months. Contact Transfusion Medicine for confirmation.
  • Neonate: Infants less than 4 months of age require a group and screen tested once during initial hospital admission. Contact Transfusion Medicine for confirmation.
  • Saskatoon Pre-admission Clinic testing may be valid up to 28 days providing that the patient has not been transfused or pregnant in the last 3 months. Contact Transfusion Medicine for confirmation.
  • Group specific red blood cells will be issued when:
    • a blood group result is completed on the current admission
      AND
    • a second specimen is collected and tested (for blood group confirmation) or a historical blood group is available
  • Group O red cells will be issued until two patient ABO/Rh group results are available (from two separate specimens)
  • Prenatal screens where the pregnancy is terminated, only Rh negative patients require antibody screens
  • In pregnant mothers, Prenatal Group and Antibody Screens are performed by  Canadian Blood Services: https://blood.ca/sites/default/files/Diagnostic_Services/SK/CBS-SK-Request-for-Perinatal-Testing.pdf
SHR LIS Test Code​
  • ​BBSPC (For Laboratory Use Only)

Test Ordering Requirements​ ​

​Forms Required

Patient History​ ​

​Relevant Clinical History
  • ​Recent transfusion history (within the last 3 months)
  • Recent pregnancy (within the last 3 months)
  • Prior hematopoietic progenitor stem cell transplant
  • Previously identified antibodies (If patient presents with an Antibody Card, photocopy card and forward it along with the test request form)

 

​If you choose to print this information, it is valid only on date of print.

Laboratory Controlled Document LSM-46 v3

Last Modified: Wednesday, August 16, 2017 |
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