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Pathology and Laboratory Medicine
Test Catalogue

​Immunoglobulin Gene Rearrangement
Multiplex PCR Assay
(IGH heavy and/or kappa light chain B-cell clonality)

Human Molecular Genetics


​ ​Specimen Requirements

Type​
  • ​Submit only one of the following specimens as appropriate:
    • Bone marrow aspirate
    • Peripheral blood
    • Body fluid
    • Fresh or frozen tissue
    • Formalin fixed, paraffin embedded tissue
​Container

​Bone marrow aspirate:

Peripheral blood:

Body fluid:

  • Preferred Collection Container: sterile tube, vial, container

Fresh or frozen tissue:

  • Preferred Collection Container: clean container

Formalin fixed, paraffin embedded tissue:

  • Preferred Collection Container:  paraffin block
​Collection Procedure
  • ​Collect as appropriate for specimen type
  • Fresh specimens should be transported to Royal University Hospital, Human Molecular Genetics without unnecessary delay
​Required Volume

Bone marrow aspirate: 

  • Optimal Volume:  1.0 ml

Peripheral blood :

  • Optimal Volume:  1.0 ml

Body fluid:

  • Optimal Volume:  1.0 ml

Fresh or frozen tissue:

  • Optimal Volume:  100.0 mg
​Stability/Storage
  • ​Ship all but frozen specimens at ambient temperature
  • Bone marrow, peripheral blood and fluids may be stored at 2 - 8°C
Grounds for Rejection​

Testing Information​ ​

Availability​
  • ​Testing available Monday to Friday, 8:00 a.m. to 4:30 p.m.
  • STAT testing can be requested but is only available Monday to Friday
Testing Site​Royal University Hospital, Human Molecular Genetics Laboratory
Results Reporting​
  • ​Results are generally available within 7 working days
  • STAT results are generally available within 3 working days
​Methodology
  • ​Fluorescently tagged primers that target the conserved framework and the joining
    regions of the immunoglobulin heavy chain and the variable and joining regions of
    the immunoglobulin kappa light chain are used in multiplex polymerase chain
    reaction (PCR) and then size separated by capillary electrophoresis
​Clinical Interpretation
  • ​A Pathology report will be generated by the Hematopathologist
​Specimen Retention
  • ​Peripheral blood retained for 14 days
  • Bone marrow retained for 1 month
  • Paraffin blocks are stored for 20 years (Anatomic Pathology guidelines)
  • Fresh tissues and fluids are retained for 1 week
  • Extracted DNA stored for 2 years
​Additional Comments
  • ​Successful PCR is dependent on the yield and quality of the extracted DNA
  • Occasionally, and particularly in paraffin-embedded tissues, DNA yield and quality
    may not be adequate for successful testing
Clearance Required
  • ​Requests for IGH/IGK gene clonality analysis must be made after consultation with a
    Hematopathologist



​If you choose to print this information, it is valid only on date of print.

Laboratory Controlled Document LSM-336 v3

Last Modified: Monday, September 9, 2019 |
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