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​T Cell Receptor Gamma and/or Beta Gene Rearrangement Multiplex PCR Clonality Assay (TCRγ and/or TCRβ Gene Clonality Assay)

Human Molecular Genetics

​ ​Specimen Requirements

  • ​Submit only one of the following specimens as appropriate:
    • Bone marrow aspirate
    • Peripheral blood
    • Body fluid
    • Fresh or frozen tissue
    • Formalin fixed, paraffin embedded tissue

Bone marrow aspirate:

Peripheral blood:

Body fluid:

  • Preferred Collection Container: sterile tube, vial, container

Fresh or frozen tissue:

  • Preferred Collection Container: clean container

Formalin fixed, paraffin embedded tissue:

  • Preferred Collection Container:  paraffin block
​Collection Procedure
  • ​Collect as appropriate for specimen type
  • Fresh specimens should be transported to Royal University Hospital, Human Molecular Genetics without unnecessary delay
​Required Volume

​Bone marrow aspirate:

  • Optimal Volume:  1.0 ml

Peripheral blood :

  • Optimal Volume:  1.0 ml

Body fluid:

  • Optimal Volume:  1.0 ml

Fresh or frozen tissue:

  • Optimal volume:  100.0 mg
  • ​Ship all but frozen specimens at ambient temperature
  • Bone marrow, peripheral blood and fluids may be stored at 2 - 8°C
Grounds for Rejection​

Testing Information​ ​

  • ​Testing available Monday to Friday, 8:00 a.m. to 4:30 p.m.
  • STAT testing can be requested but is only available Monday to Friday
Testing Site​Royal University Hospital, Human Molecular Genetics Laboratory
Results Reporting​
  • ​Results are generally available within 7 working days
  • STAT results are generally available within 3 working days
  • ​Fluorescently tagged primers that target the conserved framework and the joining
    regions are used in multiplex polymerase chain reaction (PCR) and then size
    separated by capillary electrophoresis
​Clinical Interpretation
  • ​A Pathology report will be generated by the Hematopathologist
​Specimen Retention
  • ​Peripheral blood retained for 14 days
  • Bone marrow retained for 1 month
  • Paraffin blocks are stored for 20 years (Anatomic Pathology guidelines)
  • Fresh tissues and fluids are retained for 1 week
  • Extracted DNA stored for 2 years
​Additional Comments
  • ​Successful PCR is dependent on the yield and quality of the extracted DNA
  • Occasionally, and particularly in paraffin-embedded tissues, DNA yield and quality
    may not be adequate for successful testing
Clearance Required
  • ​Requests for TCR gene clonality analysis must be made after consultation with a

​If you choose to print this information, it is valid only on date of print.

Laboratory Controlled Document LSM-339 v3

Last Modified: Monday, September 9, 2019 |
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