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Pathology and Laboratory Medicine
Cerebrospinal Fluid (CSF) Collection Guidelines
Collect in Tube #1-4​ Laboratory Test Request ​Volume Required for Optimal Testing​ ​
​Adults and Children​Neonates


Special Testing or for Storage​ ​ ​ ​ ​ ​ ​ ​ ​

Cryptococcal Antigen​​1.0 mL​Not Applicable
Fungal Culture​​3.0 mL
Viral PCR​​1.0  mL
​West Nile​1.0 mL
​Acanthamoeba1.0 mL​
Creutzfeldt-Jakob​​3.0 mL
AFB Smear & Culture​​3.0 mL
​Electrophoresis​3.0 mL
Flow Cytometry​7.0 mL



​Culture & Sensitivity​1.0 mL​0.5 mL



​1.5 mL​0.5 mL



​Malignant Cells​3.0 mL​Not Applicable



  1. Indicate if the CSF sample is known or suspected for Creutzfeldt-Jacob Disease (CJD). Securely tighten container and place in biohazard bag. Place completed requisition in outside pouch.
  2. Hematology will determine testing to be performed from Tube #1; may be contaminated or contain excess blood.


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Laboratory Controlled Document LSM-758 v1

Last Modified: Wednesday, February 3, 2016 |
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