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Pathology and Laboratory Medicine
Minimum Whole Blood Volumes Pediatric

​Minimum Whole Blood Volumes for Pediatric Collections


This document provides information regarding the minimum volume of whole blood to collect on neonate, pediatric or other patients that are hard to collect blood from. When the minimum volume is collected, repeat or reflex testing may not be possible.


A collection shall not exceed recommended blood draw limit of 1.7 mL/kg of weight per day for a child unless the physician has completed the "Unusual Phlebotomy Authorization Form" (SHA Form # 103066)

​General Chemistry Test

  • The following chart of routine chemistry tests can all be analyzed on 0.5 mL of blood for all or any combination
  • Collect 0.5 mL of blood in a LIGHT GREEN tube or GREEN microtainer
  • Additional wshole blood will be required if the patients hematocrit is greater than 40 L/L
  • Refer to Pediatric Minimum Volume Test Requirements Table "LSM-847" for additional testing requirements

Routine Chemistry Tests

​Bilirubin (total and direct)
​LYTE6 - Sodium Potassium Chloride
TCO2 Urea Creatinine​

​Blood Gas and Metabolite Tests Including Lactate

  • For Arterial Specimens collect 0.6 mL in a Balanced Heparinized Syringe on ice
  • For Capillary specimens collect 1 full (0.125 mL) Balanced Heparinized Capillary tube on ice. (Hgb and Hematocrit are not reportable)
  • For venous specimens collect 0.6 mL in a Balanced Heparinized Syringe or dark green tube on ice​

​Immunosuppressant's (Cyclosporine, Tacrolimus, Sirolimus)

  • Collect 0.5 mL for the first test and 0.3 mL for each additional test​

General Hematology Tests​

  • For routine coagulation testing, collect a pediatric sodium citrate/blue tube filled to minimum draw line
  • For CBC/Retic/Manual differential collect 0.5 mL in an EDTA/Lavender tube or microvette​


If you choose to print this information, it is valid only on date of print.

Laboratory Controlled Document LSM-421 v3

Last Modified: Wednesday, June 23, 2021 |
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