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Pathology and Laboratory Medicine
Test Catalogue
Monospot

Blood

Clinical Microbiology

​Ordering Recommendations

  • Use this test to aid in diagnosis of Infectious Mononucleosis (acute Epstein-Barr virus infection)
  • List any relevant clinical symptoms and illness duration

​Specimen Requirements​

Type​
  • ​Serum
​Container
  • ​Preferred Collection Container: Gold (gel)
  • Alternative Collection Container: Red
​Required Volume
  • Optimal Volume: 8 mL
  • Minimum Volume: 2 mL
    (Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory/reflex testing. In some situations, a minimum volume may require a second collection)
​Stability/Storage
  • Separate Serum from blood as soon as possible to avoid hemolysis
  • refrigerate at 2 to 8°C for up to 3 days
  • > 3 days freeze at -20°C
Grounds for Rejection​

Testing Information​ ​

Availability​
Testing Site​
  • Clinical Microbiology Laboratory, RUH
Results Reporting​
  • 1 to 3 days for routine samples 
  • Stat results within 2 hours of collection
​Methodology
  • ​membrane strip based on immunoassay
​Clinical Interpretation
  • Results should be interpreted along with patient history
  • Positive result generally indicates a recent infection however, occasionally the antibody can persist for months
  • for patients between 4 and 12 years, Monospot sensitivity is lower than for adults. This means a negative monospot result in a 4 to 12 year old patients do not represent a true negative result. Negative Monospot results in these patients must be confirmed by EBV serology)
​Specimen Retention
  • 1 month

Test Ordering Requirements​

Forms Required

 

​If you choose to print this information, it is valid only on date of print.

Laboratory Controlled Document LSM-444 v3

Last Modified: Friday, May 3, 2019 |
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